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Lipase – catalyzed formation of pentyl nonanoate using screened immobilized lipase from Rhizomucor meihei


Muniandy, Megala and Lasekan, Ola and Mohd Ghazali, Hasanah and Abdul Rahman, Mohd Basyaruddin (2019) Lipase – catalyzed formation of pentyl nonanoate using screened immobilized lipase from Rhizomucor meihei. Brazilian Journal of Chemical Engineering, 36 (3). pp. 1089-1097. ISSN 0104-6632: EISSN: 1678-4383


Bio-catalysis has attracted the special attention of industrial flavour producers in the production of valuable ester compounds. In this study, the synthesis of pentyl nonanoate ester (a short chain ester with fruity aroma) was carried out with a commercial immobilized lipase from Rhizomucor meihei Lipozyme (RMIM) as biocatalyst in the esterification reaction between nonanoic acid and pentanol. Various reaction parameters such as enzyme concentration, substrate concentration, reaction temperature and reaction time in solvent-free system were screened to enhance the ester formation with the best yield. A maximum yield for pentyl nonanoate (86.08 %) in a solvent-free system was obtained within 150 min, at a reaction temperature of 45 OC, molar ratio of 1:9 M, amount of enzyme of 0.2 g, water addition of 0.2 % v/v and shaking speed of 150 rpm. This work suggests that pentyl nonanoate ester can be produced in a very high yield and in a short period by lipase-catalysed reactions of nonanoic acid and pentanol, using immobilized lipase from RMIM (lipase from Rhizomucor miehei immobilized on anionic exchange support).

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Science
DOI Number: https://doi.org/10.1590/0104-6632.20190363s20180419
Publisher: Brazilian Society of Chemical Engineering
Keywords: Pentyl nonanoate; Immobilized enzyme (RMIM); Solvent free system
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 26 Sep 2021 21:07
Last Modified: 26 Sep 2021 21:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1590/0104-6632.20190363s20180419
URI: http://psasir.upm.edu.my/id/eprint/81235
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