UPM Institutional Repository

Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration


Subbiah, Suresh Kumar and Teh, Seoh Wei and Koh, Avin Ee Hwan and Alsaeedi, Hiba Amer and Lam, Chen Shen and Abd Rashid, Munirah and Harun, Mohd Hairul Nizam and Mohd Saleh, Muhamad Fakhri and Luu, Chi D. and Ng, Min Hwei and Mohd Isa, Hazlita and Leow, Sue Ngein and Then, Kong Yong and Bastion, Mae-Lynn Catherine and Mok, Pooi Ling and Muthuvenkatachalam, Bala Sundaram and Samrot, Anthony V. and Swamy, K. B. and Nandakumar, Jaikumar (2019) Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration. Journal of Photochemistry and Photobiology, B-Biology, 198. pp. 1-8. ISSN 1011-1344


Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3 × 105 DPSCs as well as a single systemic administration of NaIO3 (40 mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4 weeks, which was statistically significant (* P ≤ .05) compared to the control. Retinal thickness of the control was also found to be thinner (*** P ≤ .001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration.

Download File

[img] Text (Abstract)

Download (74kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.jphotobiol.2019.111561
Publisher: Elsevier
Keywords: Dental pulp stemcells; Photoreceptors; Retinal pigment epithelium; Retinal degeneration; Sodium iodate
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 06 Nov 2020 19:10
Last Modified: 06 Nov 2020 19:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jphotobiol.2019.111561
URI: http://psasir.upm.edu.my/id/eprint/81092
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item