UPM Institutional Repository

Co-culture systems for the production of secondary metabolites: current and future prospects


Zin, Quat Tan and Hui, Yin Leow and Lee, David Charles Weerasingam and Kanakeswary, Karisnan and Song, Adelene Ai Lian and Chun, Wai Mai and Wai, Sum Yap and Swee, Hua Erin Lim and Kok, Song Lai (2019) Co-culture systems for the production of secondary metabolites: current and future prospects. Open Biotechnology Journal, 13. pp. 18-26. ISSN 1874-0707


Microorganisms are the great sources of Natural Products (NPs); these are imperative to their survival apart from conferring competitiveness amongst each other within their environmental niches. Primary and secondary metabolites are the two major classes of NPs that help in cell development, where antimicrobial activity is closely linked with secondary metabolites. To capitalize on the effects of secondary metabolites, co-culture methods have been often used to develop an artificial microbial community that promotes the action of these metabolites. Different analytical techniques will subsequently be employed based on the metabolite specificity and sensitivity to further enhance the metabolite induction. Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography (GC)-MS are commonly used for metabolite separation while Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) have been used as tools to elucidate the structure of compounds. This review intends to discuss current systems in use for co-culture in addition to its advantages, with discourse into the investigation of specific techniques in use for the detailed study of secondary metabolites. Further advancements and focus on co-culture technologies are required to fully realize the massive potential in synthetic biological systems.

Download File

[img] Text
MICRO.pdf - Published Version

Download (5kB)
Official URL or Download Paper: https://benthamopen.com/FULLTEXT/TOBIOTJ-13-18

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.2174/1874070701913010018
Publisher: Bentham Open
Keywords: Co-culture systems; Secondary metabolites; Synthetic biology; Cell to cell interactions; Metabolomics; Metabolite profiling
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 15 Oct 2020 13:03
Last Modified: 15 Oct 2020 13:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2174/1874070701913010018
URI: http://psasir.upm.edu.my/id/eprint/80952
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item