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Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium


Fadzil, Nadia Izati and Ahmad, Siti Aqlima and Yasid, Nur Adeela and Sabullah, Mohd Khalizan and Mohd Daud, Hassan and Khalid, Ariff and Padrilah, Siti Nadzirah and Shukor, Mohd Yunus (2019) Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium. Journal of Environmental Biology, 40 (22). pp. 133-142. ISSN 0254-8704; ESSN: 2394-0379


Aim : Cholinesterase (ChE) inhibition and histopathological features of brain tissues of Clarias gariepinus were investigated with a purpose to develop biomarkers for monitoring heavy metals. Methodology : The effects of cadmium toxicity on were assessed by ChE inhibition and characterisation of ChE by affinity chromatography and histological variations in brain tissues were studied by H&E staining under light microscope. Results : The purified enzyme gave 10.58 fold purification with the recovery of 12% with specific activity of 159.729 U-1 mg . The Michaelis-Menten constant (K ) m and V value obtained was 1.985 mM max -1 -1 and 0.4479 µmol min mg , respectively. The enzyme showed the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylcholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE showed highest activity at 35°C in Tris-HCl pH 7.5. The metal inhibition study also found the maximal inhibitory concentration (IC ) 50 -1 value for cadmium to be 0.6517 mg l . Histological study revealed decrease in the number of cells, degeneration of Purkinje cells, increased aggregation area and cells necrosis. Interpretation : Inhibition of cholinesterase activity and degeneration of Purkinje cells observed in the present study can be used as a tool for further developing a biomarker for detecting heavy metals in the aquatic ecosystems. C. gariepinus

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.22438/jeb/40/2/MRN-815
Publisher: Triveni
Keywords: Biomarker; Cadmium; Cholinesterase; Clarias gariepinus; Histopathology
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 15 Oct 2020 22:59
Last Modified: 15 Oct 2020 22:59
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.22438/jeb/40/2/MRN-815
URI: http://psasir.upm.edu.my/id/eprint/80897
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