UPM Institutional Repository

Central composite design as a tools for optimization of antioxidant activity on cocoa shell extract


Md Yusof, Arief Huzaimi and Abd Gani, Siti Salwa and Zaidan, Uswatun Hasanah and Halmi, Mohd Izuan Effendi (2019) Central composite design as a tools for optimization of antioxidant activity on cocoa shell extract. International Journal of Engineering and Advanced Technology, 8 (6S3). pp. 169-173. ISSN 2249-8958


This study seeks to analyze and optimize the antioxidant activity thru beta carotene bleaching assay influenced by different extraction condition on extracts obtained from Malaysia Cocoa Shell. The condition was optimized by response surface methodology with five levels of the model. There are three variables involved in this study, namely: ethanol concentration (70-90 v/v %), temperature (45-65 ºC), and ultrasound irradiation time (30-60 min). The optimum condition obtains for temperature, duration of extraction and solvent concentration were 55 ºC, 45 minutes and 63.18% respectively at a constant frequency of 40 kHz. In this condition, the antioxidant activity experimental was 98.91±0.5% and predicted 98.44%. The ANOVA shows the coefficient of determination (R2) and the lack of fit test was 0.9846 and 0.6105 respectively. The desirability function from T-test data, modeled equation fits the data, reveals that the design can be used to predicting future observation within the design range.

Download File

[img] Text
CENTRAL.pdf - Published Version

Download (5kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.35940/ijeat.F1028.0986S319
Publisher: Blue Eyes Intelligence Engineering & Sciences Publication
Keywords: Antioxidant; Cocoa shells and response surface methodology
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 15 Oct 2020 13:17
Last Modified: 15 Oct 2020 13:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.35940/ijeat.F1028.0986S319
URI: http://psasir.upm.edu.my/id/eprint/80876
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item