Citation
Yaacob, Nor Suhaila and Abdullah, Hasdianty and Meegala, Nallapan Maniyam and Ahmad, Siti Aqlima and Azwan, Hazeeq Hazwan and Mohamad, Rosfarizan and Ariff, Arbakariya
(2019)
Biotransformation using resting cells of Rhodococcus UKMP-5M for phenol degradation.
Biocatalysis and Agricultural Biotechnology, 21.
pp. 1-6.
ISSN 1878-8181
Abstract
Phenol is a toxic compound that may be transformed into non-toxic compounds by the activity of microbial cells. The possibility of using biotransformation method for the degradation of phenol was studied using the whole cells of Rhodococcus UKMP-5M suspended in 250 mL shake flask with buffered liquid containing phenol. The cells of Rhodococcus UKMP-5M were produced by cultivation in Minimal Salt Medium (MSM) with the addition of phenol and/or glucose as carbon source. The biotransformation conditions to obtain the highest percentage of phenol degradation were as follows; pH 7.4, 0.5 g/L phenol in MSM as biotransformation medium, cells were produced by cultivation in MSM supplemented with 0.5 g/L phenol and the optimal cell concentration was 10%. The phenol degradation rate obtained in biotransformation using Rhodococcus UKMP-5M cells correlated well with phenol hydroxylase activity. The highest percentage of phenol degradation in biotransformation using suspended cells of Rhodococcus UKMP-5M was only up to 89%, which was slightly lower than those obtained in growing cell system (98%).
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