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Effects of auxin and cytokinin on callus induction in Catharanthus roseus (L.) G. Don


Abdul Rahman, Natrisya Nur and Rosli, Rozita and Kadzimin, Saleh and Hakiman, Mansor (2019) Effects of auxin and cytokinin on callus induction in Catharanthus roseus (L.) G. Don. Fundamental and Applied Agriculture, 4 (3). pp. 928-932. ISSN 2518-2021; ESSN: 2415-4474


The study was conducted to observe the effect of different concentration and combination of auxin and cytokinin towards the callus induction of C. roseus. Explants comprising of basal leaf with petioles of Catharanthus roseus were cultured onto MS media supplemented with different types and concentrations of auxins (naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic (2,4- D)) and cytokinins (benzyl amino purine (BAP), and kinetin). Calli produced from explants showed differences in response in each of the treatment combinations. Treatments with kinetin and NAA, BAP with 2,4-D (Experiment B) did not differ significantly. Treatment with 3.0 mg L−1 BAP + 3.0 mg L−1 NAA (Experiment C) gave the highest dry weight (2.776 g) suggesting an optimum level of combination for callus induction.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Medicine and Health Science
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.5455/faa.54779
Publisher: Farm to Fork Foundation
Keywords: Catharanthus roseus (L.) G. Don; Callus induction; Auxin; Cytokinin
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 15 Oct 2020 22:40
Last Modified: 15 Oct 2020 22:40
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5455/faa.54779
URI: http://psasir.upm.edu.my/id/eprint/80788
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