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Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE


Citation

Halmi, Mohd Izuan Effendi and Kassim, Adibah and Abd. Shukor, Mohd. Yunus (2019) Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE. Rendiconti Lincei. Scienze Fisiche e Naturali, 30 (3). pp. 358-601. ISSN 1720-0775

Abstract

Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of several heavy metals using a 15-min assay format. The assessment was carried out by exposing strain MIE to different concentrations of heavy metals ranging from 0.001 to 200 mg/L in a DTX microplate 96 wells. The toxicity result based on the inhibitory concentration (IC50) was Hg (0.053 mg/L) > Ag (0.12 mg/L) > Cu (0.85 mg/L) > Ni (12.32 mg/L) > Zn (18.72 mg/L) > Cr (26.02 mg/L). Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) analyses showed the sensitivity (IC50) of strain MIE to several toxic heavy metals are comparable to the commercial luminescent assay, Microtox™ as both clusters together making it a good choice for an alternative near-real-time monitoring of heavy metals. The sensitivity of strain MIE towards heavy metals was proven through field trial works on several heavy metal-polluted sites in Malaysia. Thus, it is a good candidate as an early detection system for heavy metals in aquatic bodies in tropical countries.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Springer
Keywords: Wastewater; Photobacterium sp.; Heavy metals
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 23 Jan 2021 21:05
Last Modified: 23 Jan 2021 21:05
URI: http://psasir.upm.edu.my/id/eprint/80742
Statistic Details: View Download Statistic

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