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Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection


Khalid, Bahariah and Mazlan, Nur-Fadhilah and Tan, Ling Ling and Heng, Lee Yook and Abdul Karim, Nurul Huda and Jamaludin, Nur Diyana and Mohd Yusof, Nurul Yuziana and Huai, Doris Xia Quay (2019) Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection. Talanta, 198. pp. 358-370. ISSN 0039-9140; ESSN: 1873-3573


An optical genosensor based on Schiff base complex (Zn2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10–15 M to 1 × 10−3 M with a low limit of detection (LOD) obtained at 1.21 × 10–16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.talanta.2019.02.036
Publisher: Elsevier
Keywords: Acrylic microsphere; Dengue virus; Optical biosensor; Reflectance spectrophotometer; Salphen
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 09 Nov 2020 15:30
Last Modified: 09 Nov 2020 15:30
Altmetrics: http://www.altmeric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.talanta.2019.02.036
URI: http://psasir.upm.edu.my/id/eprint/80534
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