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Intestinal permeability and transport of apigenin across caco-2 cell monolayers


Zulkifli, Marina and Ismail, Amin and Loh, Su Peng and Jailani, Fadhilah and Kassim, Nur Kartinee (2019) Intestinal permeability and transport of apigenin across caco-2 cell monolayers. Journal of Food Bioactives, 7. pp. 48-55. ISSN 2637-8752; ESSN: 2637-8779


Investigation on bioavailability was carried out by determining the absorption and transport of bioaccessible apigenin from Mangifera indica (Water Lily var.) into Caco-2 human intestinal cell using a reliable and sensitive analytical method of LC-MS/MS. Results revealed that the concentration of glucuronidated apigenin lower than apigenin. The apigenin was metabolised inside the cells through glucuronidation process, and cross the monolayer to reach the basolateral sides or effluxed back to the apical side. The permeability coefficient of apigenin from apical to basolateral sides and basolateral to apical sides showed a medium permeability was less than 20 x10-6 cm.sec-1. Since the value of efflux ratio was 1.5, it suggested that the apigenin was absorbed and transported through a simple diffusion mechanism.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.31665/JFB.2019.7198
Publisher: International Society for Nutraceuticals and Functional Foods
Keywords: Bioavailability; Permeability coefficient; Apigenin; LC-MS/MS
Depositing User: Azhar Abdul Rahman
Date Deposited: 21 Oct 2020 18:30
Last Modified: 21 Oct 2020 18:30
Altmetrics: http://altmetrics.com-details.php?domain=psair.upm.edu.my&doi=10.31665/JFB.2019.7198
URI: http://psasir.upm.edu.my/id/eprint/80311
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