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Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms


Citation

A. H., Muhamad Afiq and Shaharuddin, Noor Azmi and R., Zubaidah (2019) Identification of reliable reference genes for gene expression studies of oil palm plantlets using normfinder and bestkeeper algorithms. Journal of Oil Palm Research, 31 (2). pp. 1-8. ISSN 1511-2780

Abstract

Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy. Gene expression profiles of particular genes in different tissues and developmental stages aid in the understanding of their biological functions. However, the accuracy and reliability of qRT-PCR to study gene expression demand the use of species-specific reference genes for normalisation. Therefore, appropriate reference genes for qRT-PCR need to be determined for oil palm ( Elaeis guineensis) tissues. In this study, six candidate reference genes namely actin, Cyp2, GRAS, EgEfa1, F-box, and GLU were examined in leaf, node, internode, root, cabbage, shoot primordial and basal stem tissues of two-year old clonal oil palms. Two statistical algorithms, NormFinder and BestKeeper verified the stability of GRAS and Cyp2 reference genes in the oil palm tissues tested. The combination of Cyp2 and GRAS reference genes are recommended for gene expression normalisation profiles in nurserystage oil palm plantlets.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.21894/jopr.2019.0010
Publisher: Malaysian Palm Oil Board
Keywords: Normalisation; Oil palm; Quantitative real-time (qRT); Reference gene
Depositing User: Azhar Abdul Rahman
Date Deposited: 23 Sep 2020 08:04
Last Modified: 23 Sep 2020 08:04
Altmetrics: http://altmetrics.com-details.php?domain=psair.upm.edu.my&doi= 10.21894/jopr.2019.0010
URI: http://psasir.upm.edu.my/id/eprint/80136
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