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Identification of nitric oxide inhibitory compounds from the rhizome of Curcuma xanthorrhiza


Awin, Tahani M. and Buzgaia, Nawal and Abd Ghafar, Siti Zulaikha and Mediani, Ahmed and Mohd Zaudzi, Siti Munirah and Maulidiani, M. and Shaari, Khozirah and Abas, Faridah (2019) Identification of nitric oxide inhibitory compounds from the rhizome of Curcuma xanthorrhiza. Food Bioscience, 29. pp. 126-134. ISSN 2212-4292; ESSN: 2212-4306


Curcuma xanthorrhiza is a natural source of traditional medicines with numerous biological properties. This study profiles the metabolites in different fractions (hexane, chloroform, ethyl acetate and methanol) of the ethanolic extracts of C. xanthorrhiza using a nuclear magnetic resonance-based metabolomics approach. The nitric oxide (NO) inhibitory activity was determined using a Griess assay. The bioactivity and phytochemical constituents were correlated using partial least squares regression that indicated the differences between the 4 solvents based on the identified metabolites and their bioactivities. The results showed that the ethyl acetate fractions of C. xanthorrhiza had the highest NO inhibitory activity, this high activity is associated with the large quantity of curcuminoids found in this fraction. The metabolites that are responsible for the bioactivity are curcumin, demethoxycurcumin, l-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)-6-heptene-3,5-dione, and 1-(4- hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione. Ultra-performance liquid chromatography–tandem mass spectrometry was used to identify more metabolites in the active fraction, including bisdemethoxycurcumin and 1,7-bis(4-hydroxy-3-methoxyphenyl)-heptane-3,5-diol. The findings suggested that the ethyl acetate fraction of C. xanthorrhiza showed significant activity and can be a potential natural source of NO inhibitors.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.fbio.2019.04.009
Publisher: Elsevier BV
Keywords: C. xanthorrhiza; Metabolomics; Nitric oxide inhibition; Temu lawak; Jamu
Depositing User: Azhar Abdul Rahman
Date Deposited: 23 Sep 2020 08:01
Last Modified: 23 Sep 2020 08:01
Altmetrics: http://altmetrics.com-details.php?domain=psair.upm.edu.my&doi= 10.1016/j.fbio.2019.04.009
URI: http://psasir.upm.edu.my/id/eprint/80135
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