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Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21


Abels, Erik R. and Maas, Sybren L. N. and Nieland, Lisa and Wei, Zhiyun and Cheah, Pike See and Tai, Eric and Kolsteeg, Christy-Joy and Dusoswa, Sophie A. and Ting, David T. and Hickman, Suzanne and El Khoury, Joseph and Krichevsky, Anna M. and Broekman, Marike L. D. and Breakefield, Xandra O. (2019) Glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular miR-21. Cell Reports, 28 (12). pp. 3105-3119. ISSN 2211-1247


Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.celrep.2019.08.036
Publisher: Cell Press
Keywords: Glioblastoma; Microglia; Gliomas; Brain tumors
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 30 May 2023 08:42
Last Modified: 30 May 2023 08:42
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.celrep.2019.08.036
URI: http://psasir.upm.edu.my/id/eprint/79998
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