UPM Institutional Repository

Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma


Citation

Lee, Yu Zhao and Lee, Ming Tatt and Lajis, Nordin and Sulaiman, Mohd Roslan and Israf Ali, Daud Ahmad and Tham, Chau Ling (2012) Development and validation of a bioanalytical method for quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma. Molecules, 17 (12). pp. 14555-14564. ISSN 1420-3049

Abstract

A sensitive and accurate high performance liquid chromatography with ultraviolet/visible light detection (HPLC-UV/VIS) method for the quantification of 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) in rat plasma was developed and validated. BHMC and the internal standard, harmaline, were extracted from plasma samples by a simple liquid–liquid extraction using 95% ethyl acetate and 5% methanol. Plasma concentration of BHMC and internal standard were analyzed by reversed phase chromatography using a C18 column (150 × 4.6 mm I.D., particle size 5 µm) and elution with a gradient mobile phase of water and methanol at a flow rate of 1.0 mL/min. Detection of BHMC and internal standard was done at a wavelength of 380 nm. The limit of quantification was 0.02 µg/mL. The calibration curves was linear (R2 > 0.999) over the concentration range of 0.02–2.5 µg/mL. Intra- and inter-day precision were less than 2% coefficient of variation. The validated method was then applied to a pharmacokinetic study in rats by intravenous administration of BHMC at a single dose of 10 mg/kg. Pharmacokinetic parameters such as half-life, maximum plasma concentration, volume of distribution, clearance and elimination rate constant for BHMC were calculated.


Download File

[img] Text
78036.pdf
Restricted to Repository staff only

Download (343kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/molecules171214555
Publisher: MDPI
Keywords: BHMC; Curcuminoid analogue; HPLC; Pharmacokinetic; Plasma extraction
Depositing User: Nabilah Mustapa
Date Deposited: 03 May 2020 22:32
Last Modified: 03 May 2020 22:32
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules171214555
URI: http://psasir.upm.edu.my/id/eprint/78036
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item