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BDMC33, a curcumin derivative suppresses inflammatory responses in macrophage-like cellular system: role of inhibition in NF-κB and MAPK signaling pathways


Citation

Lee, Ka Heng and Chow, Yuh Lit and Vidyadaran, Sharmili and Abas, Faridah and Mohammed Alitheen, Noorjahan Banu and Shaari, Khozirah and Israf Ali, Daud Ahmad and Lajis, Nordin and Ahmad, Syahida (2012) BDMC33, a curcumin derivative suppresses inflammatory responses in macrophage-like cellular system: role of inhibition in NF-κB and MAPK signaling pathways. International Journal of Molecular Sciences, 13 (3). pp. 2985-3008. ISSN 1661-6596; ESSN: 1422-0067

Abstract

Our preliminary screening has shown that curcumin derivative BDMC33 [2,6-bis(2,5-dimethoxybenzylidene)cyclohexanone] exerted promising nitric oxide inhibitory activity in activated macrophages. However, the molecular basis and mechanism for its pharmacological action is yet to be elucidated. The aim of this study was to investigate the anti-inflammatory properties of BDMC33 and elucidate its underlying mechanism action in macrophage cells. Our current study demonstrated that BDMC33 inhibits the secretion of major pro-inflammatory mediators in stimulated macrophages, and includes NO, TNF-α and IL-1β through interference in both nuclear factor kappaB (NF-κB) and mitogen activator protein kinase (MAPK) signaling cascade in IFN-γ/LPS-stimulated macrophages. Moreover, BDMC33 also interrupted LPS signaling through inhibiting the surface expression of CD-14 accessory molecules. In addition, the inhibitory action of BDMC33 not only restricted the macrophages cell (RAW264.7), but also inhibited the secretion of NO and TNF-α in IFN-γ/LPS-challenged microglial cells (BV-2). The experimental data suggests the inflammatory action of BDMC33 on activated macrophage-like cellular systems, which could be used as a future therapeutic agent in the management of chronic inflammatory diseases.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.3390/ijms13032985
Publisher: MDPI
Keywords: Anti-inflammatory; BV-2; Curcumin; MAPK; NF-κB; RAW 264.7
Depositing User: Nabilah Mustapa
Date Deposited: 01 Jun 2020 09:04
Last Modified: 01 Jun 2020 09:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ijms13032985
URI: http://psasir.upm.edu.my/id/eprint/78007
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