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Single locus microsatellite development for the Malaysian giant freshwater prawns, Macrobrachium rosenbergii


Citation

Bhassu, Subha and Hassan, Rosly and Yusoff, Khatijah and Jamari, Zainoddin and Siraj, Siti Shapor and Tan, Soon Guan (2005) Single locus microsatellite development for the Malaysian giant freshwater prawns, Macrobrachium rosenbergii. Pertanika Journal of Tropical Agricultural Science, 28 (2). pp. 87-94. ISSN 1511-3701; ESSN: 2231-8542

Abstract / Synopsis

Prawns of the genus Macrobrachium Bate, 1868 (Crustacea: Palaemonidae) are a highly diverse group of decopod crustaceans found in circumtropical marine-, estuarine- and freshwaters. At present, wild stocks have been used to cultivate commercial cultures in many areas in Malaysia. Such repeated harvesting from wild stocks, however, will eventually lead to extinction and loss of genetic diversity. Therefore, it is important to know the genetic make up of these wild stocks in order to manage the populations for conservation and breeding purposes. In the present study, a novel method known as Random Amplified Microsatellites (RAMS) was used to develop single locus microsatellite markers. A total of 13 microsatellite regions were detected. The findings of this study showed a successful detection of the microsatellite sequences in M. rosenbergii using RAMS. This study will be continued to identify more clones that contain microsatellite sequences and to design the forward and reverse primers for each microsatelite region. M. rosenbergii sequences were homologous with Danio rerio (Zebra fish) and Perna viridis (green lipped mussel).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher: Universiti Putra Malaysia Press
Keywords: Genetic diversity; M. rosenbergii microsatellites
Depositing User: Muizzudin Kaspol
Date Deposited: 02 Nov 2015 10:24
Last Modified: 02 Nov 2015 10:24
URI: http://psasir.upm.edu.my/id/eprint/7775
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