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Diversity of Trichoderma species isolated from soil


Syd Abdul Rahman, Sharifah Siti Maryam and Abd Aziz, Nor Azwady and Mohd Zainudin, Nur Ain Izzati (2016) Diversity of Trichoderma species isolated from soil. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 35).


Soils consist of complex mixtures of mineral, water, air, organic matter and as an ecosystem of a large number of countless microorganisms such as bacteria, fungi and nematodes. Due to the limitation on understanding of fungi biodiversity in soil, many people do not know there consists of very useful microbe that can help in suppression of plant pathogens. Trichoderma (Hypocreales, Ascomycota) is one of the microorganisms that living in all types of soil. Trichoderma species has been used as a biological control agent of phytopathogens due to its abundance and having an antagonistic property. This study will provide checklists of Trichoderma species that have been isolated from cultivated crop soils in Malaysia. The objectives of this study are to isolate and identify Trichoderma species from soil based on morphological characterization and to calculate diversity of Trichoderma species based on Shannon-Wiener Index. Soil samples from each cultivated agricultural crops were collected in three spots in 17 locations around Malaysia. Isolation of Trichoderma was completed using soil dilution technique to obtain a pure single colony-forming unit (CFU). A total of 253 of Trichoderma isolates were successfully isolated from rhizosphere of different crops. All isolates were tentatively identified into six species such as T. harzianum (114 isolates), T. asperellum (65 isolates), T. hamatum (22 isolates), T. viride (26 isolates),T. koningii (20 isolates) and T. virens (6 isolates).The differences between species were characterized based on their colony features, pigmentation on potato dextrose agar (PDA) and the branching of conidiophores. The diversity index of Trichoderma species found in the Malaysia soil was 1.43 and T. harzianum was dominated. Due to diverse species of Trichoderma found in Malaysia, it will increase the opportunity in selection for biological control agent of plant diseases.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Science
Publisher: Department of Biology, Faculty of Science, Universiti Putra Malaysia
Keywords: Trichoderma species; Soil fungi; Biological control; Diversity
Depositing User: Nabilah Mustapa
Date Deposited: 15 Apr 2020 16:44
Last Modified: 15 Apr 2020 16:44
URI: http://psasir.upm.edu.my/id/eprint/77496
Statistic Details: View Download Statistic

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