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Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals.


Citation

Lee, Hai Yen and Chai, Lay Ching and Tang, Sui Yan and Selamat, Jinap and Mohammad Ghazali, Farinazleen and Radu, Son (2009) Application of MPN-PCR in biosafety of Bacillus cereus s.l. for ready-to-eat cereals. Food Control, 20 (11). pp. 1068-1071. ISSN 0956-7135

Abstract / Synopsis

Since Bacillus cereus is one of the important foodborne pathogens, it is interesting to investigate the biosafety of Bacillus spp. and B. cereus in ready-to-eat cereals marketed local supermarkets. For this investigation, the prevalence and enumeration of Bacillus spp. and B. cereus were assayed using MPN-PCR method. Results showed that 78% of the processed cereal products intended for direct consumption were positive for the presence of B. cereus with concentrations ranging from as low as 30 MPN/g to more than 24,000 MPN/g. The concentration obtained from this study also reflects on the differences in the contamination level between the infant food, raw cereals, cereal bars, ready-to-eat breakfast cereals and pre-mixed drinks examined. Hence, application of the MPN-PCR method was found to be useful to address the biosafety concerns of B. cereus in ready-to-eat cereals.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.foodcont.2009.01.009
Publisher: Elsevier
Keywords: Biosafety, Prevalence, Enumeration, Bacillus cereus, Ready-to-eat cereals
Depositing User: Samsida Samsudin
Date Deposited: 19 Aug 2010 15:32
Last Modified: 05 Oct 2015 13:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2009.01.009
URI: http://psasir.upm.edu.my/id/eprint/7742
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