UPM Institutional Repository

Evaluation of antagonism and lytic enzymes production by Trichoderma species as potential biocontrol agents against Rigidoporus microporus


Go, Wen Ze and H'ng, Paik San and Wong, Mui Yun (2018) Evaluation of antagonism and lytic enzymes production by Trichoderma species as potential biocontrol agents against Rigidoporus microporus. In: Joint Symposium of the 8th International Agriculture Congress 2018 and 6th International Symposium for Food & Agriculture 2018 (8th IAC – 6th ISFA 2018), 13-15 Nov. 2018, Auditorium Rashdan Baba, TNCPI Building, Universiti Putra Malaysia. (pp. 273-277).


Species of the genus Trichoderma are well known as the biocontrol against many plants diseases. Adoption of biological control methods as an alternative disease control measure has become a top priority these days due to the frequent use of agrochemicals that lead to environmental pollution and development of chemical resistance. A total of four Trichoderma species namely, T. koningiopsis (HT001), T. spirale (HT009), T. asperellum (ST011) and T. reesei (ST013) were isolated from rhizosphere soils of healthy and symptomatic tree area to test for their ability to inhibit the white root rot pathogen, Rigidoporus microporus in rubber tree. The strain with high antagonistic potential was determined mainly by plate assay method on each of the respective solid media. Among all, T. asperellum revealed a significant high antagonistic ability against R. microporus as compared to other strains of Trichoderma on the direct confrontation plate assay with the percentage of inhibition recorded at 93.02%. Besides that, the production of chitinase and cellulase enzymes were notable from the plate assay of T. asperellum with the formation of purple colour zone on chitinase detection plate and yellow-opaque halo zone on the cellulase detection plate. The strain was also able to produce β-1,3-glucanase in the culture medium supplied with chitin as a carbon source. These enzymes are believed to facilitate the cell wall-degradation process during the mycoparasitic activity. The above ability possessed by T. asperellum suggests that it a best candidate for the biocontrol against white root rot pathogen, R. microporus.

Download File

[img] Text
8TH IAC – 6TH ISFA 2018-54.pdf
Restricted to Repository staff only

Download (361kB)

Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Agriculture
Faculty of Forestry
Institute of Plantation Studies
Institute of Tropical Forestry and Forest Products
Publisher: Faculty of Agriculture, Universiti Putra Malaysia
Keywords: Trichoderma; Biocontrol agents; Rigidoporus microporus
Depositing User: Nabilah Mustapa
Date Deposited: 05 Mar 2020 06:29
Last Modified: 05 Mar 2020 06:29
URI: http://psasir.upm.edu.my/id/eprint/77375
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item