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Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29


Citation

Azlan, Azrina and Ismail, Maznah and Abdul Hamid, Azizah (2008) Extraction and determination of oryzanol in rice bran of mixed Herbarium UKMB; AZ 6807: MR 185, AZ 6808: MR 211, AZ6809: MR 29. ASEAN Food Journal, 15 (1). pp. 89-96. ISSN 0127-7324; ESSN: 1505-5337

Abstract

The level of total lipid and oryzanol content, an important antioxidant compound in locally produced bran was investigated. Total lipid in rice bran was extracted using 3:2 chloroform:methanol mixture yielding 16.4% fat. Oryzanol content was determined without saponification using a reverse-phase HPLC. Four fractions of oryzanol were successfully separated and quantitated. The 4 isomers were cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, campestryl ferulate and mixtures of β–sitosteryl ferulate and cycloartanyl ferulate. The oryzanol content of local mixed varieties ranged from 23.7–43.0 mg g-1. The oryzanol concentration may depend on factors such as plant varieties, processing methods employed, extracting solvent used and ratio of extracting solvent to bran as well as extracting solvent temperatures. This study showed the potential of oryzanol extract from rice bran as a source of antioxidant.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Publisher: Universiti Putra Malaysia Press
Notes: Continued by: International Food Research Journal
Keywords: Vitamin; Oryzanol; Rice bran; Food analysis
Depositing User: Yusfauhannum Mohd Yunus
Date Deposited: 26 Nov 2008 12:54
Last Modified: 15 Nov 2016 07:14
URI: http://psasir.upm.edu.my/id/eprint/773
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