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Molecular investigation of feline coronavirus (FCoV) in local pet cats


Liew, Wuan Hoong and Abd. Rahaman, Nor Yasmin and Mummoorthy, Kunambiga and Arshad, Siti Suri and Omar, Abdul Rahman and Anand, Prem and Karunanithi, Kiven Kumar (2019) Molecular investigation of feline coronavirus (FCoV) in local pet cats. Jurnal Veterinar Malaysia, 31 (2). pp. 13-18. ISSN 0128-2506; ESSN: 2682-9339


Feline coronavirus (FCoV) infection is a very common in cat population. FCoV is further classified into two biotypes namely feline enteric coronavirus (FECV) and mutated feline infectious peritonitis virus (FIPV), in which FIPV causes a fatal immune complex disease by changing the tropism from enterocytes to monocytes. Previous studies on molecular detection of FCoV in cats were carried out in catteries but limited study investigate the presence of FCoV antigen in local pet cats. By considering this fact, this study aims to detect FCoV antigen via RT-PCR assay in local pet cats and to compare the similarity of the identified FCoV strain with previous related virus by phylogenetic analysis. By using convenience sampling, rectal swabs and buffy coat were collected from 16 clinically ill pet cats and 5 healthy pet cats. Viral RNA was extracted and subjected to one-step RT-PCR, targeting polymerase gene. Only one out of 21 fecal samples was positive for FCoV and none from buffy coat samples. Phylogenetic analysis revealed that the identified positive sample was highly homologous, up to 95%, to FCoV strain from Netherlands and South Korea on partial sequence of polymerase gene. In conclusion, this study detected FCoV antigen in local pet cats from fecal samples while negative detection from fecal and buffy coat samples could not completely rule out the possibilities of FCoV infection due to the complexity of the virus diagnosis that require multiple series of analysis.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Veterinary Association Malaysia
Keywords: Feline coronavirus (FCoV); Phylogenetic analysis; Reverse transcription polymerase chain reaction (RT-PCR)
Depositing User: Nabilah Mustapa
Date Deposited: 05 Feb 2020 04:23
Last Modified: 05 Feb 2020 04:23
URI: http://psasir.upm.edu.my/id/eprint/76610
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