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Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex


Citation

Abdullah Halid, Nurul Izni and Hasbullah, Siti Aishah and Ahmad, Haslina and Lee, Yook Heng and Abd Karim, Nurul Huda and Harun, Siti Norain (2015) Electrochemical DNA biosensor for detection of porcine oligonucleotides using [Ru(bpy)2PIP]2+ complex. In: Third International Conference on Advances in Bio-Informatics, Bio-Technology and Environmental Engineering (ABBE 2015), 26-27 May 2015, Birmingham, United Kingdom. (pp. 15-17).

Abstract / Synopsis

An electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) complexruthenium(II) complex, [Ru(bpy)2(PIP)]2+, (bpy = 2,2’bipyridine, PIP = 2-phenylimidazo[4,5-f[[1,10-phenanthroline]) as label redox have been developed. The study was carried out by immobilization of porcine aminated DNA probes sequences on screen printed electrode (SPE) modified with succinimide-acrylic microspheres and [Ru(bpy)2(PIP)]2+ to detect DNA hybridization event. The electrochemical detection by redox active ruthenium (II) complex was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicate that the interaction of [Ru(bpy)2(PIP)]2+ with hybridization complementary DNA has higher response compared to single-stranded and mismatch complementary DNA. Under optimized condition, this porcine DNA biosensor shows linear response range towards target DNA within range of 1 x10 -5 uM to 1x10-13 uM.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Science
DOI Number: https://doi.org/10.15224/978-1-63248-060-6-04
Publisher: Institute of Research Engineers and Doctors, USA
Keywords: DNA biosensor; Ruthenium; Electrochemical
Depositing User: Nabilah Mustapa
Date Deposited: 07 Oct 2019 12:13
Last Modified: 07 Oct 2019 12:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.15224/978-1-63248-060-6-04
URI: http://psasir.upm.edu.my/id/eprint/75445
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