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Semisynthesis and in vitro anticancer activities of andrographolide analogues


Jada, Srinivasa Rao and Subur, Genevieve Suseno and Matthews, Charlie and Hamzah, Ahmad Sazali and Lajis, Md Nordin and Saad, Mohammad Said and Stevens, M. F. G. and Stanslas, Johnson (2007) Semisynthesis and in vitro anticancer activities of andrographolide analogues. Phytochemistry, 68 (6). pp. 904-912. ISSN 0031-9422


The plant Andrographis paniculata found throughout Southeast Asia contains Andrographolide 1, a diterpenoid lactone, which has antitumour activities against in vitro and in vivo breast cancer models. In the present study, we report on the synthesis of andrographolide derivatives, 3,19-isopropylideneandrographolide (2), 14-acetyl-3,19-isopropylideneandrographolide (3) and 14-acetylandrographolide (4), and their in vitro antitumour activities against a 2-cell line panel consisting of MCF-7 (breast cancer cell line) and HCT-116 (colon cancer cell line). Compounds 2 and 4 were also screened at the US National Cancer Institute (NCI) for their activities against a panel of 60 human cancer cell lines derived from nine cancer types. Compound 2 was found to be selective towards leukaemia and colon cancer cells, and compound 4 was selective towards leukaemia, ovarian and renal cancer cells at all the dose-response parameters. Compounds 2 and 4 showed non-specific phase of the cell cycle arrest in MCF-7 cells treated at different intervals with different concentrations. NCI’s COMPARE and SOM mechanistic analyses indicated that the anticancer activities of these new class of compounds were not similar to that of standard anticancer agents, suggesting novel mechanism(s) of action.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1016/j.phytochem.2006.11.031
Publisher: Elsevier
Keywords: Andrographis paniculata, Acanthaceae, Andrographolide, Semisynthesis, Anticancer, Cell cycle
Depositing User: Najwani Amir Sariffudin
Date Deposited: 20 Jul 2010 07:05
Last Modified: 20 Oct 2015 04:07
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.phytochem.2006.11.031
URI: http://psasir.upm.edu.my/id/eprint/7522
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