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Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana


Citation

Chon, Bernard Han Ho and Mustapa Kamal, Siti Mazlina and Danquah, Michael K. and Harun, Razif (2018) Optimization of sub-critical water extraction (SWE) of lipid and eicosapentaenoic acid (EPA) from Nannochloropsis gaditana. Biomed Research International, 2018. art. no. 8273581. pp. 1-11. ISSN 2314-6133; ESSN: 2314-6141

Abstract

Microalgae are a promising source of omega-3. The purpose of this study was to extract lipid with a relatively high content of eicosapentaenoic acid (EPA) from Nannochloropsis gaditana using subcritical water extraction (SWE). The effects of different temperatures (156.1-273.9°C), extraction times (6.6-23.4 minutes), and biomass loadings (33-117 g algae/L) on the extraction yield were studied. From the optimization study using central composite design (CCD), quadratic models generated for lipid yield and EPA composition were considered to be significant models (p < 0.05). The predictive equations were also formed for lipid yield and EPA composition. The predicted optimum lipid yield and EPA composition at 236.54°C, 13.95 minutes, and 60.50 g algae/L were 18.278 wt% of total biomass and 14.036 wt% of total fatty acid methyl ester (FAME), respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.1155/2018/8273581
Publisher: Hindawi
Keywords: Microalgae; Omega-3; Eicosapentaenoic acid (EPA); Nannochloropsis gaditana
Depositing User: Mr. Sazali Mohamad
Date Deposited: 16 Jul 2020 07:08
Last Modified: 16 Jul 2020 07:08
Altmetrics: http://www.altmetric.com/details.php?domain=psair.upmedu.my&doi= 10.1155/2018/8273581
URI: http://psasir.upm.edu.my/id/eprint/75167
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