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Label-free dengue E protein detection using a functionalized tapered optical fiber sensor


Citation

Mustapha Kamil, Yasmin and Abu Bakar, M. H. and Mustapa, Mohd Azmir and Yaacob, Mohd Hanif and Zainol Abidin, Nadiah Husseini and Syahir, Amir and Lee, Huijing and Mahdi, Mohd Adzir (2018) Label-free dengue E protein detection using a functionalized tapered optical fiber sensor. Sensors and Actuators B: Chemical, 257. 820 - 828. ISSN 0925-4005; ESSN: 1873-3077

Abstract

The surge of Dengue cases around the globe has intensified the demand for a reliable diagnostic method. The work demonstrates, to the best of our knowledge, the first label-free optical based sensor for detection of Dengue II E proteins. This was achieved by utilizing tapered optical fiber that has been functionalized with complementary recombinant antibodies. The fundamental concept of the sensor relies on the interaction between strong evanescent waves resulting from the dimensional change of the fiber and immune complex formed on the surface of the fiber when the virus is present. Sensitivity and detection limit values obtained with the sensor setup are 5.02 nm/nM and 1 pM, respectively, with a standard deviation value of no more than ±0.4. The compact and rapid sensor is a viable alternative for label-free and quantitative assessment of the infection, which may assist in providing better clinical management and understanding of the disease.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
DOI Number: https://doi.org/10.1016/j.snb.2017.11.005
Publisher: Elsevier
Keywords: Dengue E protein; Tapered optical fiber; Dengue sensor; Optical sensor; Fiber optic
Depositing User: Mr. Sazali Mohamad
Date Deposited: 05 Dec 2019 06:54
Last Modified: 05 Dec 2019 06:54
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.snb.2017.11.005
URI: http://psasir.upm.edu.my/id/eprint/74812
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