UPM Institutional Repository

Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract


Citation

Leng, Wei Khoo and Siew, Audrey Foong Kow and M., Maulidiani and Lee, Ming Tatt and Tan, Chin Ping and Shaari, Khozirah and Tham, Chau Ling and Abas, Faridah (2018) Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract. Molecules, 23 (9). art. no. 2172. pp. 1-18. ISSN 1420-3049

Abstract

The present study aims for the first time to provide the in vivo acute toxicological profile of the highest dose of Clinacanthus nutans (Burm. f.) Lindau water leaf extract according to the Organization for economic co-operation and development (OECD) 423 guidelines through conventional toxicity and advanced proton nuclear magnetic resonance (1H-NMR) serum and urinary metabolomics evaluation methods. A single dose of 5000 mg/kg bw of C. nutans water extract was administered to Sprague Dawley rats, and they were observed for 14 days. Conventional toxicity evaluation methods (physical observation, body and organ weight, food and water consumption, hematology, biochemical testing and histopathological analysis) suggested no abnormal toxicity signs. Serum 1H-NMR metabolome revealed no significant metabolic difference between untreated and treated groups. Urinary 1H-NMR analysis, on the other hand, revealed alteration in carbohydrate metabolism, energy metabolism and amino acid metabolism in extract-treated rats after 2 h of extract administration, but the metabolic expression collected after 24 h and at Day 5, Day 10 and Day 15 indicated that the extract-treated rats did not accumulate any toxicity biomarkers. Importantly, the outcomes further suggest that single oral administration of up to 5000 mg/kg bw of C. nutans water leaf extract is safe for consumption.


Download File

[img] Text (Abstract)
Hematological, biochemical, histopathological and 1H-NMR metabolomics application in acute toxicity evaluation of clinacanthus nutans water leaf extract.pdf

Download (9kB)
Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/30158427/

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.3390/molecules23092172
Publisher: MDPI
Keywords: Clinacanthus nutans; Medicinal plant; Biochemical test; Histopathology; Urinary metabolomics; Toxicity; 1H-NMR metabolomics; Metabolic pathway
Depositing User: Mr. Sazali Mohamad
Date Deposited: 16 Oct 2020 05:59
Last Modified: 16 Oct 2020 05:59
Altmetrics: http://www.altmetric.com/details.php?domain=psair.upmedu.my&doi=10.3390/molecules23092172
URI: http://psasir.upm.edu.my/id/eprint/74501
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item