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Putative iron acquisition systems in Stenotrophomonas maltophilia


Citation

Vasodavan, Kalidasan and Azman, Adleen and Joseph, Narcisse and Subbiah, Suresh Kumar and Awang Hamat, Rukman and Neela, Vasantha Kumari (2018) Putative iron acquisition systems in Stenotrophomonas maltophilia. Molecules, 23 (8). art. no. 2048. pp. 1-19. ISSN 1420-3049; ESSN: 1420-3049

Abstract

Iron has been shown to regulate biofilm formation, oxidative stress response and several pathogenic mechanisms in Stenotrophomonas maltophilia. Thus, the present study is aimed at identifying various iron acquisition systems and iron sources utilized during iron starvation in S. maltophilia. The annotations of the complete genome of strains K279a, R551-3, D457 and JV3 through Rapid Annotations using Subsystems Technology (RAST) revealed two putative subsystems to be involved in iron acquisition: the iron siderophore sensor and receptor system and the heme, hemin uptake and utilization systems/hemin transport system. Screening for these acquisition systems in S. maltophilia showed the presence of all tested functional genes in clinical isolates, but only a few in environmental isolates. NanoString nCounter Elements technology, applied to determine the expression pattern of the genes under iron-depleted condition, showed significant expression for FeSR (6.15-fold), HmuT (12.21-fold), Hup (5.46-fold), ETFb (2.28-fold), TonB (2.03-fold) and Fur (3.30-fold). The isolates, when further screened for the production and chemical nature of siderophores using CAS agar diffusion (CASAD) and Arnows's colorimetric assay, revealed S. maltophilia to produce catechol-type siderophore. Siderophore production was also tested through liquid CAS assay and was found to be greater in the clinical isolate (30.8%) compared to environmental isolates (4%). Both clinical and environmental isolates utilized hemoglobin, hemin, transferrin and lactoferrin as iron sources. All data put together indicates that S. maltophilia utilizes siderophore-mediated and heme-mediated systems for iron acquisition during iron starvation. These data need to be further confirmed through several knockout studies.


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Official URL or Download Paper: https://www.mdpi.com/1420-3049/23/8/2048

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3390/molecules23082048
Publisher: MDPI
Keywords: NanoString technologies; RAST server; Stenotrophomonas maltophilia; Iron acquisition systems; Iron-depleted; Siderophores
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 09 May 2020 18:33
Last Modified: 09 May 2020 18:33
Altmetrics: https://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules23082048
URI: http://psasir.upm.edu.my/id/eprint/73686
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