UPM Institutional Repository

Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals


Citation

Gunasekaran, Baskaran and Wan Johari, Wan Lutfi and Wasoh @ Mohamad Isa, Helmi and Masdor, Noor Azlina and Abd. Shukor, Mohd Yunus (2018) Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals. Bioremediation Science and Technology Research, 6 (1). pp. 9-13. ISSN ESSN: 2289-5892

Abstract

Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment. In this work, a preliminary study was carried out on the applicability of various local plants as a source of protease for the future development of the inhibitive enzyme assay for heavy-metals. The crude proteases preparation was assayed using casein as a substrate in conjunction with the Coomassie dye-binding assay. The crude protease from the kesinai plant was found to be the most potent plant protease. The crude enzyme exhibited broad temperature and pH ranges for activity and will be developed in the future as a potential inhibitive assay for heavy metals.


Download File

[img] Text (Abstract)
Preliminary screening of plant proteases as a potential source for the development of an inhibitive assay for heavy metals.pdf

Download (8kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Environmental Studies
Publisher: Hibiscus Publisher
Keywords: Crude protease; Plant protease; Kesinai; Casein; Coomassie dye binding assay
Depositing User: Nida Hidayati Ghazali
Date Deposited: 08 Jun 2020 18:14
Last Modified: 08 Jun 2020 18:14
URI: http://psasir.upm.edu.my/id/eprint/73602
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item