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Chemical and genetic markers for identification of honey origin from its bee speciation


Chin, Nyuk Ling and Kek, Siok Peng (2018) Chemical and genetic markers for identification of honey origin from its bee speciation. International Journal of Food Engineering, 4 (4). 304 - 307. ISSN 1556-3758


The need for accurate and reliable methods for identification of honey origin is important for reducing honey fraud. This study has identified suitable chemical and genetic markers to determine the origin of honey from its bee source of Apis honey bees or Trigona stingless bee. In the chemical analysis, moisture, fructose, glucose, sucrose, free acidity, and colour intensity were chemical markers identified for differentiating honey by its bee origin. The use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) on honey composition have successfully classified honey into groups of Apis and Trigona. In the DNA-based method, mitochondrial cytochrome c oxidase subunit I (COI) gene was used as a genetic marker to identify honey origin by its bee species accurately from the clear groupings and distinct clusters in phylogenetic trees. The genetic marker of COI gene is accurate and reliable for this identification as it has direct matching to its reference bee species. Incorporating both chemical and genetic markers affirm the identity of honey.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
DOI Number: https://doi.org/10.18178/ijfe.4.4.304-307
Publisher: Walter de Gruyter GmbH
Keywords: Honey; Entomological origin; Chemical composition; Pattern recognition; DNA marker; Phylogenetic analysis
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 09 Nov 2020 15:12
Last Modified: 09 Nov 2020 15:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.18178/ijfe.4.4.304-307
URI: http://psasir.upm.edu.my/id/eprint/73386
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