UPM Institutional Repository

Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers


Siew, Ging Yang and Ng, Wei Lun and Tan, Sheau Wei and Mohammed Alitheen, Noorjahan Banu and Tan, Soon Guan and Yeap, Swee Keong (2018) Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers. PeerJ, 6. pp. 1-18. ISSN 2167-8359


Durian (Durio zibethinus) is one of the most popular tropical fruits in Asia. To date, 126 durian types have been registered with the Department of Agriculture in Malaysia based on phenotypic characteristics. Classification based on morphology is convenient, easy, and fast but it suffers from phenotypic plasticity as a direct result of environmental factors and age. To overcome the limitation of morphological classification, there is a need to carry out genetic characterization of the various durian types. Such data is important for the evaluation and management of durian genetic resources in producing countries. In this study, simple sequence repeat (SSR) markers were used to study the genetic variation in 27 durian types from the germplasm collection of Universiti Putra Malaysia. Based on DNA sequences deposited in Genbank, seven pairs of primers were successfully designed to amplify SSR regions in the durian DNA samples. High levels of variation among the 27 durian types were observed (expected heterozygosity, HE = 0.35). The DNA fingerprinting power of SSR markers revealed by the combined probability of identity (PI) of all loci was 2.3×10-3. Unique DNA fingerprints were generated for 21 out of 27 durian types using five polymorphic SSR markers (the other two SSR markers were monomorphic). We further tested the utility of these markers by evaluating the clonal status of shared durian types from different germplasm collection sites, and found that some were not clones. The findings in this preliminary study not only shows the feasibility of using SSR markers for DNA fingerprinting of durian types, but also challenges the current classification of durian types, e.g., on whether the different types should be called "clones", "varieties", or "cultivars". Such matters have a direct impact on the regulation and management of durian genetic resources in the region.

Download File

[img] Text
Genetic variation and DNA .pdf

Download (11kB)
Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/29511604/

Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.7717/peerj.4266. eCollection 2018
Publisher: PeerJ
Keywords: DNA fingerprinting; Durio zibethinus; Genetic diversity; Microsatellite markers
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Mar 2021 15:52
Last Modified: 15 Mar 2021 15:53
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.7717/peerj.4266. eCollection 2018
URI: http://psasir.upm.edu.my/id/eprint/72837
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item