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Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda)


Citation

Ng, Chew Hee and Ong, Alan Han Kiat and Kong, Chiak Wu and Tan, Kong Wai and Raja Abdul Rahman, Raja Noor Zaliha and Mohd Yamin, Bohari and Ng, Seik Weng (2006) Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). Polyhedron, 25 (16). pp. 3118-3126. ISSN 0277-5387; ESSN: 1873-3719

Abstract / Synopsis

M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV–visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na2H2EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.poly.2006.05.020
Publisher: Elsevier
Keywords: (N,N′-ethylenediaminediacetato)metal(II); DNA cleavage; Crystal structure; Nucleolytic efficiency; Hydrogen peroxide
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 15 Jun 2010 02:10
Last Modified: 26 Apr 2016 08:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.poly.2006.05.020
URI: http://psasir.upm.edu.my/id/eprint/7270
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