Simple Search:

Isolation of salinity tolerant genes from the mangrove plant, Bruguiera cylindrica by using suppression subtractive hybridization (SSH) and bacterial functional screening


Citation

Wong, Yeen Yee and Ho, Chai Ling and Nguyen, Phuoc Dang and Teo, Swee Sen and Harikrishna, Jennifer Ann and Abdul Rahim, Raha and Wong, Michael C. V. L. (2007) Isolation of salinity tolerant genes from the mangrove plant, Bruguiera cylindrica by using suppression subtractive hybridization (SSH) and bacterial functional screening. Aquatic Botany, 86 (2). pp. 117-122. ISSN 0304-3770

Abstract / Synopsis

In this study, we have identified and isolated 126 salinity tolerant cDNAs from the root of a mangrove plant, Bruguiera cylindrica (L.) Blume by using suppression subtractive hybridization (SSH) and bacterial functional screening. Sequencing of 51 subtracted cDNA clones that were differentially expressed in the root of B. cylindrica exposed to 20 parts per thousand (ppt) NaCl water revealed 10 tentative unique genes (TUGs) with putative functions in protein synthesis, storage and destination, metabolism, intracellular trafficking and other functions; and 9 unknown proteins. Meanwhile, the 75 cDNA sequences of B. cylindrica that conferred salinity tolerance to Escherichia coli consisted of 29 TUGs with putative functions in transportation, metabolism and other functions; and 33 with unknown functions. Both approaches yielded 42 unique sequencess that have not been reported else where to be stress related and might provide further understanding of adaptations of this plant to salinity stress.


Download File

[img]
Preview
PDF (Abstract)
Isolation of salinity tolerant genes from the mangrove plant.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.aquabot.2006.09.009
Keywords: Mangrove, Bacterial functional screening, Bruguiera cylindrica, Salinity tolerance, Sequence analysis, Suppression subtractive hybridization (SSH)
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 14 Jun 2010 07:22
Last Modified: 22 Oct 2015 02:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.aquabot.2006.09.009
URI: http://psasir.upm.edu.my/id/eprint/7262
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item