UPM Institutional Repository

Efficient in vitro multiple shoots regeneration system from rice shoot apex in recalcitrant Malaysian Indica rice cultivars (Oryza sativa L.)


Citation

Teh, Chui Yao and Mahmood, Maziah and Shaharuddin, Noor Azmi and Ho, Chai Ling (2018) Efficient in vitro multiple shoots regeneration system from rice shoot apex in recalcitrant Malaysian Indica rice cultivars (Oryza sativa L.). Indian Journal of Biotechnology, 17 (3). 504 - 513. ISSN 0972-5849; ESSN: 0975-0967

Abstract

Development of in vitro rice shoot regeneration system can contribute to new advances for the selection of stress tolerant cultivars as well as production of elite cultivar through genetic transformation approach. Multiple shoot induction was carried out in two widely cultivated Malaysian indica rice MR 220 and MR 253 using 10 mm rice shoot apex along with coleoptile isolated from 4 days old seedlings. The explants were cultured on a Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and Kinetin (KIN) at (0 – 10 mg/L) for two culture periods 2 and 4 weeks. The results showed that the optimal multiple shoots growth were observed in media supplemented with 6 mg/L KIN, which induced an average (10.30 ± 0.95) shoots for MR 220 and (6.67 ± 0.57) shoots for MR 253. Results from biochemical changes studies indicated that a relatively higher total chlorophyll and soluble protein content were obtained in rice shoots treated with KIN as compared to BAP suggesting KIN is more suitable in the in vitro multiple shoots regeneration system of Malaysian indica rice.


Download File

[img] Text
Efficient in vitro multiple shoots regeneration system .pdf

Download (18kB)
Official URL or Download Paper: http://nopr.niscair.res.in/handle/123456789/45274

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Agriculture
Publisher: CSIR-NISCAIR
Keywords: Cytokinin; in-vitro; Rice; Shoot apex; Regeneration
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 22 Oct 2020 04:49
Last Modified: 22 Oct 2020 04:49
URI: http://psasir.upm.edu.my/id/eprint/72535
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item