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Effects of ultrasound assisted extraction in conjugation with aid of actinidin on the molecular and physicochemical properties of bovine hide gelatin


Citation

Ahmad, Tanbir and Ismail, Amin and Ahmad, Siti Aqlima and Abdul Khalil, Khalilah and Leo, Teik Kee and Mohamed, Elmutaz Atta Awad and Imlan, Jurhamid Columbres and Sazili, Awis Qurni (2018) Effects of ultrasound assisted extraction in conjugation with aid of actinidin on the molecular and physicochemical properties of bovine hide gelatin. Molecules, 23 (4). pp. 1-17. ISSN 1420-3049

Abstract

Actinidin was used to pretreat the bovine hide and ultrasonic wave (53 kHz and 500 W) was used for the time durations of 2, 4 and 6 h at 60 °C to extract gelatin samples (UA2, UA4 and UA6, respectively). Control (UAC) gelatin was extracted using ultrasound for 6 h at 60 °C without enzyme pretreatment. There was significant (p < 0.05) increase in gelatin yield as the time duration of ultrasound treatment increased with UA6 giving the highest yield of 19.65%. Gel strength and viscosity of UAC and UA6 extracted gelatin samples were 627.53 and 502.16 g and 16.33 and 15.60 mPa.s, respectively. Longer duration of ultrasound treatment increased amino acids content of the extracted gelatin and UAC exhibited the highest content of amino acids. Progressive degradation of polypeptide chains was observed in the protein pattern of the extracted gelatin as the time duration of ultrasound extraction increased. Fourier transform infrared (FTIR) spectroscopy depicted loss of molecular order and degradation in UA6. Scanning electron microscopy (SEM) revealed protein aggregation and network formation in the gelatin samples with increasing time of ultrasound treatment. The study indicated that ultrasound assisted gelatin extraction using actinidin exhibited high yield with good quality gelatin.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Halal Products Research Institute
Institute of Tropical Agriculture and Food Security
DOI Number: https://doi.org/10.3390/molecules23040730
Publisher: MDPI
Keywords: Ultrasound assisted extraction; Gelatin; Actinidin; Bovine hide; Physicochemical properties; Gel strength
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 21 Oct 2020 08:10
Last Modified: 21 Oct 2020 08:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules23040730
URI: http://psasir.upm.edu.my/id/eprint/72527
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