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Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development


Adenan, Mohd Noor Hidayat and Saiful Yazan, Latifah and Christianus, Annie and Md Hashim, Nur Fariesha and Harun, Abdul Rahim and Abdul Halim, Nur Hafizati and Sani, Mohd Zulmadi and Abdul Rahim, Khairuddin (2018) Effects of Kelulut honey from Trigona sp. on zebrafish (Danio rerio) embryo that mimics human embryonic development. Journal of Cell and Animal Biology, 12 (2). pp. 5-14. ISSN 1996-0867


Kelulut honey (KH) is a type of honey with various pharmacological properties that can be found in Malaysia. Nevertheless, the safety aspects of this honey have not been adequately addressed. This study evaluated the developmental toxicity of KH from Trigona sp on zebrafish (Danio rerio) embryos. Viable zebrafish embryos at 3 hours post fertilization (hpf) (early stage) and 24 hpf (organ development stage) were treated with KH (1 to 20 mg/mL). The embryos were examined for morphological abnormalities and viability until 96 h of KH treatment. Coagulated embryos were identified after treatment with KH (≥10 mg/mL) for 3 hpf group and KH (≥12 mg/mL) for 24 hpf group. The LC50 values of KH at 96 h of exposure for the 3 hpf and 24 hpf group were 12.52 and 16.36 mg/mL, respectively. The maximum allowable concentration (MAC) for KH on 3 hpf and 24 hpf group were 0.63 and 0.82 mg/mL, respectively. The irregular cardiac rate of the embryos was noted at 10 mg/mL for 3 hpf group and 13 mg/mL of KH for 24 hpf group. In summary, the early stage embryo (3 hpf group) was more sensitive to KH than the one of later stage (24 hpf group). It indicates that serious precautions should be taken into account in the use of any material including natural product, be it food or supplement, especially in the early stage of life.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.5897/JCAB2018.0458
Publisher: Academic Journals
Keywords: Kelulut honey; Trigona sp.; Toxicity; Zebrafish (Danio rerio) embryos
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 19 Jun 2020 02:28
Last Modified: 19 Jun 2020 02:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/JCAB2018.0458
URI: http://psasir.upm.edu.my/id/eprint/72500
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