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A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene


Citation

Sulong, Moohamad Ropaning and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2006) A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene. Protein Expression and Purification, 49 (2). pp. 190-195. ISSN 1046-5928; ESSN: 1096-0279

Abstract / Synopsis

An organic solvent tolerant (OST) lipase gene from Bacillus sphaericus 205y was successfully expressed extracellularly. The expressed lipase was purified using two steps purification; ultrafiltration and hydrophobic interaction chromatography (HIC) to 8-fold purity and 32% recovery. The purified 205y lipase revealed homogeneity on denaturing gel electrophoresis and the molecular mass was at approximately 30 kDa. The optimum pH for the purified 205y lipase was 7.0–8.0 and its stability showed a broad range of pH value between pH 5.0 to 13.0 at 37 °C. The purified 205y lipase exhibited an optimum temperature of 55 °C. The activity of the purified lipase was stimulated in the presence of Ca2+ and Mg2+. Ethylenediaminetetraacetic acid (EDTA) has no effect on its activity; however inhibition was observed with phenylmethane sulfonoyl fluoride (PMSF) a serine hydrolase inhibitor. Organic solvents such as dimethylsulfoxide (DMSO), methanol, p-xylene and n-decane enhanced the activity. Studies on the effect of oil showed that the lipase was most active in the presence of tricaprin (C10). The lipase exhibited 1,3 positional specificity.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.1016/j.pep.2006.04.015
Publisher: Elsevier
Keywords: Bacteriocin release protein; Organic solvent tolerant lipase; Bacillus sphaericus 205y; Purification; Characterization
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 14 Jun 2010 01:53
Last Modified: 26 Sep 2016 07:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pep.2006.04.015
URI: http://psasir.upm.edu.my/id/eprint/7246
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