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DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14


Abdul Rahim, Raha and Hooi, Wei Yeng and Nor Shamsudin, Mariana and Radu, Son and Sankara Varma, Nadimpalli Raw and Yusoff, Khatijah (2006) DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14. Plasmid, 56 (1). pp. 53-61. ISSN 0147-619X


A small plasmid designated pAR141 was isolated from Lactococcus lactis subsp. lactis M14 and its complete 1594 base pair nucleotide sequence was determined. Analysis of the sequence indicated that this plasmid does not carry any industrially important determinants besides the elements involved in plasmid replication and control. The transcriptional repressor CopG and replication initiation protein RepB appeared as a single operon. A small countertranscribed RNA (ctRNA) coding region was found between the copG and repB genes. The double strand origin (dso) and single strand origin (sso) of rolling circle replicating (RCR) plasmids were also identified in pAR141, suggesting that this plasmid replicates by rolling circle (RC) mode. This observation was supported by S1 nuclease and Southern hybridization analyses.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.plasmid.2006.03.003
Keywords: Cryptic plasmids, Lactococcus lactis, Replication, Rolling circle replicating plasmid
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 14 Jun 2010 00:32
Last Modified: 09 Dec 2015 02:32
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.plasmid.2006.03.003
URI: http://psasir.upm.edu.my/id/eprint/7233
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