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Diagnostic approach and genetic diversity of jackfruit bronzing bacterium in Malaysia


Abidin, Nuraizat and Mohamed Zulperi, Dzarifah and Ismail, Siti Izera and Yusof, Mohd Termizi and Ismail Suhaimy, Noor Wahida and Karam Singh, Daljit Singh and Awang @ Mansor, Mohd Hakiman (2018) Diagnostic approach and genetic diversity of jackfruit bronzing bacterium in Malaysia. Asian Journal of Plant Pathology, 12 (1). 46 - 55. ISSN 1819-1541


A serious problem faced in the jack fruit industry in Malaysia caused by bronzing disease by pathogen Pantoea stewartii subspe. stewartii (P. stewartii subsp. stewartii ) as it reduces the quality of the fresh jack fruit, hence, affects the consumer preference towards the fruits. It is important to keep the fruit healthy as it provides revenue to the local and export markets in Malaysia. This review aimes to present an overview and diagnostic approach of jackfruit bronzing disease in Malaysia. Detection and identification methods following past and recent study of the causal pathogen via phenotypic identification, molecular identification, pathogenicity test, genetic relationship and genetic diversity. Successful detection and identification were obtained with appropriate phenotypic tests performed and molecular identification using CPSL1/CPSR2c primers and ES16/ESIG2c primers. Pantoea stewartii subsp. stewartii strains were also pathogenic to healthy detached and attached jack fruit and the high liability of the phylogenetic and clustering analyses in the causal pathogen via multilocus sequencing analysis (MLSA) of the partial nucleotide sequences of the genes gyrB, rpoB, atpD and infB, using a bootstrap analysis with 1000 replicates on the individual and concatenated peptide sequence trees. The presented review provides an overview on bronzing disease of jackfruit in Malaysia, the appropriate detection, identification, pathogenicity of its causal pathogen and the application of multilocus sequence analysis (MLSA) as the best tool to study the pathogen diversity and evolution. Up to now, little information and studies have been done on bronzing disease of jackfruit and its causal agent, P. stewartii subsp. stewartii in Malaysia.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3923/ajppaj.2018.46.55
Publisher: Academic Journals
Keywords: Jackfruit; Pantoea stewartii; Bronzing disease; Multilocus sequencing analysis; Pathogen diversity; Causal pathogen
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 28 Apr 2020 09:32
Last Modified: 28 Apr 2020 09:32
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajppaj.2018.46.55
URI: http://psasir.upm.edu.my/id/eprint/72280
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