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Development of isocratic RP-HPLC method for separation and quantification of L-citrulline and L-arginine in watermelons


Citation

Ridwan, Rasdin and Abdul Razak, Hairil Rashmizal and Adenan, Mohd Ilham and Md Saad, Wan Mazlina (2018) Development of isocratic RP-HPLC method for separation and quantification of L-citrulline and L-arginine in watermelons. International Journal of Analytical Chemistry, 2018. art. no. 4798530. pp. 1-9. ISSN 1687-8760; ESSN: 1687-8779

Abstract

Watermelons (Citrullus lanatus) are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% H3P04, 0.1% H3P04, and 0.7% H3P04 : ACN (90 : 10), were tested on two different columns using Zorbax Eclipse XDB-C18 and Gemini C18 with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% H3P04 on the Gemini C18 column. The method was validated and good linearity of L-citrulline and L-arginine was obtained with = 0.9956, and , , respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g), whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% H3P04 on the Gemini C18 column proved to be efficient for separation and quantification of L-citrulline and L-arginine in watermelons.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2018/4798530
Publisher: Hindawi
Keywords: Watermelons; Isocratic RP-HPLC method; L-citrulline; L-arginine
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 27 Apr 2020 05:18
Last Modified: 27 Apr 2020 05:18
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2018/4798530
URI: http://psasir.upm.edu.my/id/eprint/72263
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