UPM Institutional Repository

Design, synthesis and docking studies of Flavokawain B type chalcones and their cytotoxic effects on MCF-7 and MDA-MB-231 cell lines


Abu Bakar, Addila and Akhtar, Muhammad Nadeem and Mohd Ali, Norlaily and Yeap, Swee Keong and Quah, Ching Kheng and Loh, Wan-Sin and Mohammed Alitheen, Noorjahan Banu and Zareen, Seema and Ul-Haq, Zaheer and Shah, Syed Adnan Ali (2018) Design, synthesis and docking studies of Flavokawain B type chalcones and their cytotoxic effects on MCF-7 and MDA-MB-231 cell lines. Molecules, 23 (3). 616 - 629. ISSN 1420-3049


Flavokawain B (1) is a natural chalcone extracted from the roots of Piper methysticum, and has been proven to be a potential cytotoxic compound. Using the partial structure of flavokawain B (FKB), about 23 analogs have been synthesized. Among them, compounds 8, 13 and 23 were found in new FKB derivatives. All compounds were evaluated for their cytotoxic properties against two breast cancer cell lines, MCF-7 and MDA-MB-231, thus establishing the structure–activity relationship. The FKB derivatives 16 (IC50 = 6.50 ± 0.40 and 4.12 ± 0.20 μg/mL), 15 (IC50 = 5.50 ± 0.35 and 6.50 ± 1.40 μg/mL) and 13 (IC50 = 7.12 ± 0.80 and 4.04 ± 0.30 μg/mL) exhibited potential cytotoxic effects on the MCF-7 and MDA-MB-231 cell lines. However, the methoxy group substituted in position three and four in compound 2 (IC50 = 8.90 ± 0.60 and 6.80 ± 0.35 μg/mL) and 22 (IC50 = 8.80 ± 0.35 and 14.16 ± 1.10 μg/mL) exhibited good cytotoxicity. The lead compound FKB (1) showed potential cytotoxicity (IC50 = 7.70 ± 0.30 and 5.90 ± 0.30 μg/mL) against two proposed breast cancer cell lines. It is evident that the FKB skeleton is unique for anticancer agents, additionally, the presence of halogens (Cl and F) in position 2 and 3 also improved the cytotoxicity in FKB series. These findings could help to improve the future drug discovery process to treat breast cancer. A molecular dynamics study of active compounds revealed stable interactions within the active site of Janus kinase. The structures of all compounds were determined by 1H-NMR, EI-MS, IR and UV and X-ray crystallographic spectroscopy techniques.

Download File

[img] Text
Design, synthesis and docking studies .pdf

Download (78kB)
Official URL or Download Paper: https://www.mdpi.com/1420-3049/23/3/616

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: 0.3390/molecules23030616
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Chalcone synthesis; Breast cancer cell lines; SARs; Anti-cancer; Flavokawain B derivatives
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 29 Jun 2022 07:36
Last Modified: 29 Jun 2022 07:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules23030616
URI: http://psasir.upm.edu.my/id/eprint/72197
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item