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Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch


Citation

Wong, Chen Wai and Syed Muhammad, Sharifah Kharidah and Mat Hashim, Dzulkifly and Saari, Nazamid and Mohd Ghazali, Hasanah (2007) Enzymatic production of linear long-chain dextrin from sago (Metroxylon sagu) starch. Food Chemistry, 100 (2). pp. 774-780. ISSN 0308-8146

Abstract

Pullulanase (EC.3.2.1.41) was used to generate more linear-chain dextrin from sago starch (24.9% amylose) such that the resulting product could act as a high amylose starch. A starch suspension of 5.0% (w/v) sago starch was heated at 100 °C for 45 min and, after cooling, the gelatinized sago starch was hydrolyzed with 2.0% (v/dry weight starch) pullulanase (Promozyme 400L, Novozymes A/S, Denmark) for 24 h. The linear long-chain dextrin (LLD) content of the hydrolysate after drying, was then compared with the initial LLD content. The surface morphology of the starch granules was observed with a scanning electron microscope (SEM). The effects of gelatinization, time of reaction, pretreatment with different strengths of hydrochloric acid prior to enzyme hydrolysis, and starch and enzyme concentrations were studied. Raw sago starch was resistant to the action of pullulanase, but caused an increase in the LLD of that sago starch from an initial concentration of 24.9–33.2% following gelatinization. The best conditions to maximize the amount of LLD were 5.0% (w/v) sago starch, 2.0% (v/w) enzyme and 12 h reaction time. Acid pretreatment of the sago starch did not cause greater improvement in the accessibility and susceptibility of pullulanase as the LLD content, following pullulanase action did not change significantly. Shrinkage on the surface of the starch granules was observed with the SEM.


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Official URL or Download Paper: http://dx.doi.org/10.1016/j.foodchem.2005.10.040

Additional Metadata

Item Type: Article
Subject: Pullulanase
Subject: Sago - Hydrolysis
Subject: Dextrins
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.foodchem.2005.10.040
Publisher: Copyright © 2005 Elsevier Ltd
Keywords: Linear long-chain dextrin, Pullulanase, Sago starch, Acid hydrolysis
Depositing User: Samsida Samsudin
Date Deposited: 10 Jun 2010 03:55
Last Modified: 22 Oct 2015 02:23
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2005.10.040
URI: http://psasir.upm.edu.my/id/eprint/7173
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