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Solubility, Immunogenicity and Physical Properties Of the Nucleocapsid Protein of Nipah Virus Produced in Escherichia Coli.


Citation

Wen, Siang Tan and Swee, Tin Ong and Eshaghi, Majid and Yusoff, Khatijah and Sze, Shir Foo (2004) Solubility, Immunogenicity and Physical Properties Of the Nucleocapsid Protein of Nipah Virus Produced in Escherichia Coli. Journal of Medical Virology, 73 (1). pp. 105-112. ISSN 0146-6615

Abstract / Synopsis

The nucieocapsid (N) protein of Nipah virus (Niv) can be produced in three Escherichia coli starins (TOP10,BL (DEB) and S G935) under the control of trc promoter, However, most of the product existed in the form of insolubie inclusion bodies. There was no improvement in the solubility of the products when this protein was placed under thecontrol of T7 promoter. However, the solubility of the N protein was significantly improved by lowering the growth temperature of E. coli BL21 (DE3) cell culture. Solubility analysis of N- and C-terminally deleted mutants revealed that the full-length N protein has the highest solubility. The soluble N protein could be purified efficiently by sucrose gradient centrifugation and nickel affinity chromatography. Electron microscopic analysis of the purified product revealed that the N protein assembled into herring bone-like particles of different lengths. The C-terminal end of the N Protein contains the major antigenic region when probed with antisera from humans and pigs infected naturally.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1002/jmv.20052
Publisher: Wiley-Blackwell
Depositing User: Users 17 not found.
Date Deposited: 25 Nov 2008 13:04
Last Modified: 22 Oct 2015 02:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jmv.20052
URI: http://psasir.upm.edu.my/id/eprint/716
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