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Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay


Citation

Lee, Thong Chuan and Yusoff, Khatijah and Nathan, Sheila and Tan, Wen Siang (2006) Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay. Journal of Virological Methods, 136 (1-2). pp. 224-229. ISSN 0166-0934

Abstract / Synopsis

Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.jviromet.2006.05.017
Publisher: 2009 Elsevier B.V.
Keywords: Filamentous phage, Dot blot, NDV, Pathotypes, Phage display
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 14 Jun 2010 01:00
Last Modified: 05 Oct 2015 08:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2006.05.017
URI: http://psasir.upm.edu.my/id/eprint/7116
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