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Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester


Gunawan, Erin Ryantin and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2005) Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester. Enzyme and Microbial Technology, 37 (7). pp. 739-744. ISSN 0141-0229


The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5–10 h), temperature (30–70 °C), amount of enzyme (0.1–0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1–1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38 h, temperature 53.9 °C, amount of enzyme 0.149 g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%.

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Official URL or Download Paper: http://dx.doi.org/10.1016/j.enzmictec.2005.04.010

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1016/j.enzmictec.2005.04.010
Publisher: Elsevier
Keywords: Response surface methodology (RSM), Central composite rotatable design (CCRD), Palm oil, Lipozyme, Alcoholysis, Wax ester
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 09 Jun 2010 03:12
Last Modified: 30 Nov 2015 07:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.enzmictec.2005.04.010
URI: http://psasir.upm.edu.my/id/eprint/7106
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