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Kinetic study of lipase-catalyzed glycerolysis of palm olein using Lipozyme TLIM in solvent-free system


Choong, Thomas Shean Yaw and Yeoh, Chiou Moi and Phuah, Eng Tong and Siew, Wai Lin and Lee, Yee Ying and Tang, Teck Kim and Abdullah, Luqman Chuah (2018) Kinetic study of lipase-catalyzed glycerolysis of palm olein using Lipozyme TLIM in solvent-free system. PLOS ONE, 13 (2). art. no. e0192375. pp. 1-13. ISSN 1932-6203


Diacylglycerol (DAG) and monoacylglycerol (MAG) are two natural occurring minor components found in most edible fats and oils. These compounds have gained increasing market demand owing to their unique physicochemical properties. Enzymatic glycerolysis in solvent-free system might be a promising approach in producing DAG and MAG-enriched oil. Understanding on glycerolysis mechanism is therefore of great importance for process simulation and optimization. In this study, a commercial immobilized lipase (Lipozyme TL IM) was used to catalyze the glycerolysis reaction. The kinetics of enzymatic glycerolysis reaction between triacylglycerol (TAG) and glycerol (G) were modeled using rate equation with unsteady-state assumption. Ternary complex, ping-pong bi-bi and complex ping-pong bi-bi models were proposed and compared in this study. The reaction rate constants were determined using non-linear regression and sum of square errors (SSE) were minimized. Present work revealed satisfactory agreement between experimental data and the result generated by complex ping-pong bi-bi model as compared to other models. The proposed kinetic model would facilitate understanding on enzymatic glycerolysis for DAG and MAG production and design optimization of a pilot-scale reactor.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Institute of Bioscience
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.1371/journal.pone.0192375
Publisher: Public Library of Science
Keywords: Enzymatic glycerolysis; Lipase-catalyzed glycerolysis; Diacylglycerol; Monoacylglycerol
Depositing User: Nabilah Mustapa
Date Deposited: 10 Sep 2019 06:14
Last Modified: 10 Sep 2019 06:14
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1371/journal.pone.0192375
URI: http://psasir.upm.edu.my/id/eprint/70961
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