Citation
Adam, Aimi Zafirah
(2017)
Chemical composition, antibacterial and toxicity activities of Aquilaria leaves from three commonly planted species in Malaysia.
Masters thesis, Universiti Putra Malaysia.
Abstract
The Aquilaria genus (Thymelaeaceae), is an eminent agarwood-producing tree,
commonly found in the Indomalesia region. Illegal harvesting and random logging had
caused Aquilaria trees to be listed in the Convention on International Trade in
Endangered Species of Wild Fauna and Flora (CITES). In recent years, Aquilaria tree
cultivation at large scale has been implemented to promote sustainable agarwood
production. Besides utilizing agarwood induced in the tree trunk, the leaves can be
processed into food products such as tea, which can give some economic return while
farmers wait for their agarwood to mature. In this study, three Aquilaria species,
Aquilaria crassna, Aquilaria malaccensis and Aquilaria sinensis were selected because
they are widely planted in Peninsular Malaysia. The aim of this study was to validate the
leaf specimen of the three Aquilaria species based on morphology and molecular
identification, to identify the chemical compounds of the leaves using Gas
Chromatography-Flamed Ionization Detector (GC-FID) and Gas Chromatography-Mass
Spectrometry (GC-MS) as well as to evaluate the antibacterial and toxicity activity of
the leaves. A total of 58 compounds were obtained from the essential oils, and nine and
48 compounds from the hexane and methanol extracts, respectively. Among the major
compounds identified were hexadecanoic acid and squalene, which are known to have
antimicrobes and antioxidants properties, respectively. Methanol extracts of A.
malaccensis exhibited maximum inhibition zones against Staphylococcus aureus and
Salmonella choleraesuis. The minimum inhibitory concentration (MIC) and minimum
bactericidal concentration (MBC) of the extracts (31.25 mg/ml) produced remarkable
inhibitory effect on S. aureus compared to S. choleraesuis (250 mg/ml). The MTT test
proved that both the essential oils and hexane extracts of the three leaves had no
significant effects on the viability of the PBMCs. However, only the methanol extract of
A. malaccensis had low levels of IC50 (24.5 mg/ml) and LD50 (4537 mg/kg) values,
suggesting this species is slightly hazardous. For the comet test, the three Aquilaria
species also did not show any DNA damages (p>0.05). Similar results were obtained
when using the methanol extracts of A. crassna and A. sinensis at 2 mg/ml and 3 mg/ml, respectively. However, for the methanol extracts of A. malaccensis leaves, DNA damage
was observed (p<0.05). The tests conducted on PBMCs indicated that the essential oils
and crude extracts of Aquilaria leaves from these three species are relatively safe to
consume without major toxicity concern, but should be wisely taken in a dose-dependent
manner. This study provides a benchmark in setting up indicators for measuring toxicity
threats from the use of Aquilaria leaves, which is becoming a popular ingredient in food
or drinks preparation.
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