UPM Institutional Repository

Large scale production of liquid wax ester by immobilized lipase


Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) Large scale production of liquid wax ester by immobilized lipase. Journal of Oleo Science, 54 (4). pp. 203-209. ISSN 1345-8957; ESSN: 1347-3352


Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles.

Download File

PDF (Abstract)
Large scale production of liquid wax ester by immobilized lipase.pdf

Download (83kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.5650/jos.54.203
Publisher: Japan Oil Chemists' Society
Keywords: Liquid wax ester; Enzymatic; Esterification; Oleyl oleate
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 04 Jun 2010 06:39
Last Modified: 28 Sep 2016 03:19
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5650/jos.54.203
URI: http://psasir.upm.edu.my/id/eprint/7057
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item