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Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent


Hamidon, Noor Fazrieyana (2016) Optimization of lipase catalysed synthesis of xylose caproate ester in organic solvent. Masters thesis, Universiti Putra Malaysia.


Lipase catalysed synthesis of xylose caproate ester was performed by condensation of xylose, an aldopentose, and caproic acid in organic solvents. A dual-solvent system containing dimethylsulfoxide (DMSO) and acetone (1:10, v/v) was used to determine the optimal conditions for the reaction. Different reaction parameters (solvent system, reaction time, substrate molar ratio and the amount of enzyme loaded) were studied. The highest conversion rate (64%) was obtained within 24 hours with the optimal conditions of 16% (w/v) Novozym 435 and a molar ratio of xylose to caproic acid of 1:4 (mmol). Xylose caproate ester was purified and characterized by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FTIR), gas chromatography flame ionization (GCFID), gas chromatography mass spectrometry (GCMS) and nuclear magnetic resonance (NMR). The pure product existed as a waxy solid form with bright yellow color. The melting point, hydrophilic lipophipic value (HLB), and solubility of the product was evaluated.

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Additional Metadata

Item Type: Thesis (Masters)
Call Number: FS 2016 76
Chairman Supervisor: Emilia Abd Malek, PhD
Divisions: Faculty of Science
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 08 Sep 2021 00:42
Last Modified: 08 Sep 2021 00:42
URI: http://psasir.upm.edu.my/id/eprint/69292
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