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Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana


Ahmad, Lyena Watty Zuraine and Zainuddin, Zarina and Osman, Mohamad and Mohamad, Azhar and Jaafar Sidik, Norrizah (2019) Effects of N-methyl-N-nitrosourea on seed germination of Stevia rebaudiana. Sains Malaysiana, 48 (2). pp. 301-307. ISSN 0026-6039

Abstract / Synopsis

Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N‑methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and efficient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number:
Publisher: Penerbit Universiti Kebangsaan Malaysia
Keywords: Germination; MNU; Mutagenic effectiveness; Mutagenic efficiency; Stevia rebaudiana
Depositing User: Nabilah Mustapa
Date Deposited: 12 Jun 2019 15:44
Last Modified: 12 Jun 2019 15:44
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