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Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract


Citation

Tawai, Darreen and Noor, M. A. M. and Salleh, F. and Razak, I. S. and Mansor, R. and Ideris, A. and Md. Ajat, Mohd Mokrish (2018) Upregulation of cholesterol storage signalling genes in HEPG2 cells supplemented with edible bird nest (EBN) extract. In: 3rd World's Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2018, 18-19 Apr. 2018, Faculty of Veterinary Medicine, Universiti Putra Malaysia and Kuala Lumpur Convention Centre, Kuala Lumpur. (pp. 64-65).

Abstract

“Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed of solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression level associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. HepG2 mammalian cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there were significant differences in terms of genes expression especially in ACAT2 and LDLR genes with supplementation of EBN extract and exogenous lipid concentrate.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Faculty of Veterinary Medicine
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Edible bird nest; Swiftlet; Lipid metabolism; Genes expression
Depositing User: Nabilah Mustapa
Date Deposited: 09 Apr 2019 03:48
Last Modified: 09 Apr 2019 03:48
URI: http://psasir.upm.edu.my/id/eprint/67655
Statistic Details: View Download Statistic

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